Document Details Document Type : Thesis  Document Title : promoting wound healing activity using indian costus(saussurea lappa تحفيز التئام الجروح باستخدام القسط الهندي   Subject : Faculty of Science  Document Language : Arabic  Abstract : The plant Saussurea lappa (costus) was used in the traditional medicine to treat wounds. Although, there are no scientific reports on wound healing activity of Saussurea lappa in the literature, the present study was suggested to evaluate its scientific validity. This investigation was undertaken to determine the efficacy of Saussurea lappa on wound healing. Wound healing is the process of repair that follows injury of the skin and other soft tissues. It has three phases including inflammation, proliferative and remodeling phases. This study used the roots of Saussurea lappa to evaluate wound healing activity using tissue culture model as in vitro and excision wound model in rats as in vivo. In tissue culture, the fibroblast cells were used to investigate the effect of Saussure lappa on healing activity via their ability to stimulate fibroblast proliferation. In animal model, Excision wounds were made on the back of rats and Saussurea lappa was administered topically. Two groups of animals were used: a treated group which was treated topically with Saussurea lappa (6g/100ml water) and a control group which was left untreated. Healing was assessed by the rate of wound contraction, period of epithelialization, protein, hexosamine and hydroxyproline concentrations. The in vitro results showed that Saussurea lappa increase proliferation of fibroblasts and thus accelerate wound healing. Application of Saussurea lappa to a wound performed in rats showed that the healing process was faster than control group with out appearance of any infection. Wound contraction increased significantly in treated group compared to the control group (P<0.01). Also, the period of epithelialization decreased significantly (p<0.001). The results revealed that protein and hexosamine content in healed tissues were increased significantly in treated group compared to the control group. Increase in collagen content was observed, but not significant. As conclusion, Saussurea lappa may be reveal as safe and effective topical medicine for accelerating wound healing.  Supervisor : Dr.Wadiah Saleh Backer  Thesis Type : Master Thesis  Publishing Year : 1431 AH 2010 AD  Added Date : Saturday, December 4, 2010  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email سلافة عبدالرحمن زهران Zahran, Solafa Abdulrahman Researcher Master   Files File Name Type Description  28053.pdf pdf   Back To Researches Page